Development and characterization of a cell line from the embryos of half smooth tongue sole (Cynoglossus semilaevis)   总被引：3，自引：1，他引：2  

SHA Zhenxi  REN Guocheng  WANG Xianli  WANG N  CHEN Songlin 《海洋学报(英文版)》2010,29(2):81-87

A new cell line,CSEC,has been successfully established from embryos at gastrula stage of a cultured marine fish,half smooth tongue sole(Cynoglossus semilaevis).CSEC cells grow actively and stably more than 50 passages for over 200 d in DMEM medium supplemented with 15% FBS(fetal bovine serum),2.5 ng/cm 3 bFGF(basic fibroblast growth factor),1 ng/cm 3 LIF(leukemia inhibitory factor) and 50 mmol/dm 3 2-ME(2-mecaptoethanol).The cells grew well in the temperature range of 24-30 ℃ and the optimal growth temperature was 24℃.FBS and bFGF concentrations are the two key components for CSEC cells proliferation.Chromosome analysis reveals that CSEC cells have a normal diploid karyotype with 2n=42t.The significant fluorescent signals were observed in CSEC after transfection with the GFP reporter gene,suggesting that the CSEC cell line can be used as a useful tool for transgenic and genetic manipulation studies.CSEC cells showed the cytopathic effect(CPE) after infection with lymphosystis disease virus(LCDV) in 2 d.Moreover,the LCDV particles can be observed in the cytoplasm of virus-infected cells by electron microscopy.It suggests that CSEC could be potentially used for the study of aquatic virus.  相似文献   

Stratigraphic architecture and sedimentology of a Late Pleistocene subaqueous moraine complex,southwest Ireland     

David J. A. Evans  John F. Hiemstra  Colm Ó Cofaigh 《第四纪科学杂志》2012,27(1):51-63

Glacigenic sediments exposed in coastal cliffs cut through undulatory terrain fronting the Last Glacial Maximum laterofrontal moraine at Waterville on the Iveragh Peninsula, southwest Ireland, comprise three lithofacies. Lithofacies 1 and 2 consist of interdigitated, offlapping and superimposed ice‐proximal subaqueous outwash and stacked sequences of cohesionless and cohesive subaqueous debris flows, winnowed lag gravels and coarse‐grained suspension deposits. These are indicative of sedimentation in and around small grounding line fans that prograded from an oscillating glacier margin into a proglacial, interlobate lake. Lithofacies 3 comprises braided river deposits that have undergone significant syn‐sedimentary soft‐sediment deformation. Deposition was likely related to proglacial outwash activity and records the reduction of accommodation space for subaqueous sedimentation, either through the lowering of proglacial water levels or due to basin infilling. The stratigraphic architecture and sedimentology of the moraine at Waterville highlight the role of ice‐marginal depositional processes in the construction of morphostratigraphically significant ‘end moraine’ complexes in Great Britain and Ireland. Traditional ‘tills’ in these moraines are often crudely stratified diamictons and gravelly clinoforms deposited in ice‐proximal subaqueous and subaerial fans. Copyright © 2011 John Wiley & Sons, Ltd.  相似文献   

序贯一维型边缘检测新算法     

吕言 《武汉大学学报(信息科学版)》1988,(4)

本文提出一种既可用于从图象上提取具有某种用途的一维边缘,又可用于提取具有多种用途的二维边缘的序贯一维型边缘检测新算法。由于构成该算法重要组成部分的基础算子的作用,以及将二维问题化为一维问题求解而使其明显具有简单,快速、有效的特点。该算法还具有Abdou等所建议的边缘检测算子应具有的两个特性。  相似文献   

What has seismic missed?     

Gordon Kaufman 《Natural Resources Research》1994,3(4):304-314

The relative efficiencies of alternative geometric patterns of both discrete borehole and continuous grid line search have been extensively discussed in the mathematical geology literature. However, an equally important problem has received virtually no attention: How to use a sample of properties of geologic anomalies detected by grid line search of a region to estimate systematically both the number and size distribution of geologic anomalies missed by the search. We show how estimation methods developed in the sample survey design literature can be adapted to this problem, and we apply these methods to data describing 94 anomalies identified by a seismic reconnaissance survey.  相似文献   

地下管线探测的基本原理及解释方法研究   总被引：2，自引：0，他引：2  

封崇德  史保连 《物探与化探》1994,18(6):463-467

本文较详细地讨论了电磁直接探测法探测地下管线的基本原理和解释方法;提出了一种新的求解管线位置和深度、并给出探测结果可信度的向量交绘法。现场提供高可信度的结果;对管线探测仪器设备及应用效果作了介绍。  相似文献   

碳棒涂膜式PVC膜铯离子选择电极的研制和应用   总被引：3，自引：0，他引：3  

但德忠  谭明勇 《矿物岩石》1990,10(4):105-108

本文作者研制成功以四苯硼铯为活性物质的碳棒涂膜式PVC膜铯离子选择性电极。电极最佳膜组成:四苯硼铯5mg,邻苯二甲酸二丁酯0.4ml,硝基苯0.2ml,PVC粉0.2g。电极性能优良,制作简单,操作方便。结合大粒度磷钼酸铵和结晶形锑酸分离K~+,Na~+,巳将此电极用于卤水样中铯的测定,结果令人满意。  相似文献   

SporadicE ionization associated with meteor events     

Girija Rajaram  H Chandra 《Journal of Earth System Science》1991,100(3):255-265

Two meteor events which were sighted in the Gujarat skies of India, were accompained by the visibility of sporadicE ionization on the ionograms recorded at Ahmedabad (Geog. Lat. 23·2°N, long. 72·30°E). The first event was the Dhajala fireball which flashed into the geoatmosphere along an E-N to W-S trail at about 20·40 h IST on 28 January 1976; the closest distance of the ground projection of meteor trail from Ahmedabad was 50km. The other event was a possible meteor group sighted over Ahmedabad on 28 May 1978, at about 21·10 h IST. This work describes the nature of the sporadicE ionization observed on Ahmedabad ionograms during the two events. Features of theEs echo during the Dhajala event which indicate that it could be of meteoric origin are discussed. Meteor theory is used to relate the observed ionization with the physical dimensions of the Dhajala meteorite as obtained by other workers.  相似文献   

洱海湖盆晚第四纪地层的14C年龄测定及洱海的演变   总被引：2，自引：0，他引：2       下载免费PDF全文

彭贵  焦文强 《地震地质》1991,13(2):179-183

从大理洱海湖盆西部平原湖沼相地层的分布和相关的14C年龄测定以及文物考古资料,阐述了晚更新世中期以来洱海的演变。研究表明,距今5万年以来洱海西部古湖岸线是从西向东迁移的。期间曾有过几次摆动。引起洱海古湖岸线变迁的主导因素是点苍山断块的间歇性抬升和洱海湖盆的下沉,地质历史时期的气候干湿演变也起了重要作用  相似文献   

计算机天气图图形识别   总被引：4，自引：0，他引：4  

李振海 《气象》1994,20(6):20-23

根据天气系统的定义和实际业务中的天气图分析规范，总结出了５００、７００、８５０ｈＰａ３层高空天气图上特征等高线，特征等温线，槽线（含切变线），高（低）中心，冷（暖）中心以及热带气旋的识别方法及其判别式，并给出了实现计算机自动识别的程序设计步骤。  相似文献   

青藏高原内陆水系冰川粒雪线与中值高度趋势面的绘制与主要特征     

刘宗香  谢自楚 《冰川冻土》1995,17(4):356-359

本选用中国冰川目录数据库中的青藏高原内陆水系冰川粒雪线、中值高度和地理坐标资料，利用计算机绘图方法，绘制出冰川粒支线、中值高度在空间的变化趋势，以便全面认识该地区的冰川发育特征。  相似文献